"Because I'm involved in developing these in the U.S. where all the patients are, there are a number of studies that are amazingly successful. I have had my blood drawn more times than I can count, a colonoscopy (not as bad as I thought it would be), and two CT scans … one of those scans with a tube up my butt. Further corroborating an approach that includes both drugs is Stephan Stahl’s Facebook post: To the question, I read here somewhere what is the better effective anthelmintic Mebendazole or Joe’s used Fenbendazole. Found insideIn this book, leading experts in cancer immunotherapy join forces to provide a comprehensive guide that sets out the main principles of oncoimmunology and examines the latest advances and their implications for clinical practice, focusing ... Clioquinol inhibits the proteasome and displays preclinical activity in leukemia and myeloma. Of note, a dose of 3 mg/kg in mice translates to a dose of 0.24 mg/kg in humans, based on scaling of body weight and surface area, and appears readily achievable based on prior studies.9,11  Thus, the activity in the xenograft studies and the in vitro studies above suggests that a therapeutic window may be achievable. Ivermectin also delayed tumor growth in 3 independent mouse models of leukemia at concentrations that appear pharmacologically achievable. Clipboard, Search History, and several other advanced features are temporarily unavailable. Turmeric compounds work via a variety of mechanisms that result in cell cycle arrest or cell death. As a potential antileukemic agent, ivermectin induced cell death at low micromolar concentrations in acute myeloid leukemia cell lines and primary patient samples preferentially over normal hematopoietic cells. I may already have your interest and if so, I encourage you to check out Care Oncology Clinic out of London. 2003 Sep 15;68 (6):1089-1092. In contrast, chloride influx was not observed in DU145 cells that were resistant to 10μM ivermectin (Figure 3C and data not shown). Over 25 years of clinical experience with ivermectin: an overview of safety for an increasing number of indications. The dietary isothiocyanate, sulforaphane, targets pathways of apoptosis, cell cycle arrest, and oxidative stress in human pancreatic cancer cells and inhibits tumor growth in severe combined immunodeficient mice. (Reuters) As of Wednesday at 8 a.m. EDT, the unofficial U.S. COVID-19 … To identify known drugs with previously unrecognized antileukemia activity, we compiled and screened a library of off- and on-patent drugs for compounds cytotoxic to leukemia cells. What Conditions does IVERMECTIN Treat? But, the work underscores that the two drugs do operate differently. You must read Joe Tippen’s complete story. Given the cytotoxicity of ivermectin toward leukemia cell lines, we compared its cytotoxicity to primary normal hematopoietic cells and AML patient samples (n = 4 intermediate risk cytogenetics, n = 1 good risk cytogenetics, and n = 1 unknown cytogenetics). The Care Oncology Clinic (COC), which Jane is a staunch supporter of, includes mebendazole in their cocktail of four off-label drugs used to stave off a wide variety of cancers. and S.O.K. Cancer Free 💓. Treatment with ivermectin increased ROS production at times and concentrations that coincided with plasma membrane hyperpolarization (Figure 5A-B). For example, compared with normal neutrophils, HL60 cells overexpress the ClC-5 chloride channel that is normally expressed in renal cells.37  In support of this mechanism, we observed less chloride flux in cells more resistant to ivermectin. Differences in change of ROS compared with control were analyzed by an unpaired t test. After incubation, cell viability was measured by annexin V and PI staining. While this study is old, 1976, you can see that these two drugs are behaving differently. Now, remember that fenbendazole is not FDA approved for human consumption. Given its prior safety record in humans and animals coupled with its preclinical efficacy in leukemia, a phase 1 clinical trial could be conducted to determine the tolerance and biologic activity of oral ivermectin in these patients. Fourteen (OCI-AML2), 15 (MDAY-D2), or 17 (K562) days after injection of cells, mice were killed, tumors were excised, and the volume and mass of the tumors were measured. (D) OCI-AML2 and (E) DU145 cells were treated with 6 and 10μM ivermectin for 1 hour. ***P < .001. Blood work is normal. In Israel, quarantine may be required for vaccinated individuals who catch the Delta variant. I have read in more than one place that fenbendazole plus Vitamin E is the combination you are looking for to put the hurt on cancer cells. This book originated in numerous Gordon Research Conferences and many other meetings of scientists working in chemistry, biophysics, biochemistry, and biology related to mutagenesis and carcinogenesis. People are beating cancer at every stage. M.A.S. The hyperpolarizing impact of glycine on endothelial cells may be anti-atherogenic. Seven (AML) or 14 days (PBSCs) days after plating, the number of colonies was counted. The online version of this article contains a data supplement. Clearly Fenbendazole is the better drug. Mercuric chloride-induced reactive oxygen species and its effect on antioxidant enzymes in different regions of rat brain. The author describes the growing movement of the Repurposed Drug Revolution, and famous survivors such as Professor Ben Williams and Jane McLelland. After treatment, cell growth and viability was measured by the MTS assay. Chloride influx can increase cell size. However, when gating on the CD34+ cells from one PBSC sample, ivermectin induced cell death with an IC50 of 10.5 ± 0.6μM. Monitoring the efficacy of ivermectin and albendazole against gastro intestinal nematodes of cattle in Northern Europe. In our current study, we demonstrated that ivermectin displayed preclinical activity against hematologic malignancies in vitro and delayed tumor growth in vivo at concentrations that appear pharmacologically achievable. Proc Jpn Acad Ser B Phys Biol Sci. From this screen, we identified the antiparasitic agent, ivermectin. According to Jane, and many others, there is evidence that mebendazole reduces abnormal cell signaling (Sonic Hedgehog) which in turn helps to destroy cells associated with tumors that suppress immunity. Expression of myeloperoxidase enhances the chemosensitivity of leukemia cells through the generation of reactive oxygen species and the nitration of protein. designed research, analyzed data, and performed research; R.W. Again, a PubMed article titled, “Comparative efficacies of ivermectin, febantel, fenbendazole, and mebendazole against helminth parasites of gray foxes.” This article is from 1986 but you can see how fenbendazole was more effective than mebendazole in this particular study. MDAY-D2 mice (n = 10 per group) were treated similarly, but with escalating doses (3 mg/kg for 4 days, 5 mg/kg for 3 days, and then 6 mg/kg for 3 days) as the drug was well tolerated. Lung Scans every 3 months…I had my CTSCAN last night and My Doctor is shocked that my Scan came back shrinkage and downgraded my results to Post inflammatory! Ivermectin is most commonly used as a heartworm preventive in dogs and cats. In When Smoke Ran Like Water, the world-renowned epidemiologist Devra Davis confronts the public triumphs and private failures of her lifelong battle against environmental pollution. Data represent the mean ± SD percent colony formation compared with control treated cells. All cells were incubated at 37°C in a humidified air atmosphere supplemented with 5% CO2. Ivermectin shows excellent efficacy against conventional chemotherapy drug-resistant cancer cells … Mebendazole is rotated monthly with Doxycycline. OCI-AML2 cells were treated with increasing concentrations of ivermectin for 24 hours (A) or 6μM ivermectin for increasing times of incubation (B). Data represent the percentage of viable OCI-AML2 cells treated with the compounds (6μM) sorted in order of increasing activity. Antivirals that target the host IMPα/β1-virus interface. Ribavirin suppresses eIF4E-mediated oncogenic transformation by physical mimicry of the 7-methyl guanosine mRNA cap. On the chemical point of view please take a look at the chart below. Common side effects of ivermectin are: itching, rash, dizziness, insomnia, abdominal pain, constipation, nausea, and vomiting. And do you know that Broccoli’s famous Sulforaphane is doing its anti-cancer job in the same way as Fenbendazole does? Taken under the tongue. Sumaiya Sharmeen, Marko Skrtic, Mahadeo A. Sukhai, Rose Hurren, Marcela Gronda, Xiaoming Wang, Sonali B. Fonseca, Hong Sun, Tabitha E. Wood, Richard Ward, Mark D. Minden, Robert A. Batey, Alessandro Datti, Jeff Wrana, Shana O. Kelley, Aaron D. Schimmer; The antiparasitic agent ivermectin induces chloride-dependent membrane hyperpolarization and cell death in leukemia cells. Cell death was caspase-dependent, as cotreatment with the pan-caspase inhibitor z-VAD-fmk abrogated cell death (supplementary Figure 1A). This edition is substantially revised to reflect the major scientific advances, especially with respect to molecular genetics. Ivermectin kills the larval Onchocerca volvulus worms – microfilariae – that live in the subcutaneous tissue of an infected person. To determine whether the plasma membrane hyperpolarization observed after ivermectin treatment was related to increased chloride ion flux, we measured plasma membrane polarization after treating cells with ivermectin in buffers with and without chloride. Stage 4 perotonial cancer. 'This book gives plenty of examples of ad hominem attacks, intimidation, slander, threats of litigation, deception, dishonesty, lies and other violations of good scientific practice. Ivermectin→daunorubicin denotes that ivermectin was added initially, and the daunorubicin was added for the last 48 hours of the 72-hour experiment. They don’t prescribe these drugs. Medium was supplemented with 10% fetal bovine serum (FBS), 100 μg/mL penicillin, and 100 U/mL streptomycin (all from Hyclone). sleepiness. To identify such compounds, we compiled a small chemical library (n = 100) focused on antimicrobials and metabolic regulators with wide therapeutic windows and well-understood pharmacokinetics. This is the reason why I am taking vitamin E with mebendazole at this time. We started at 3 on and 4 off.. then went to 7 days on and 4 off. Differences in tumor volume and weight were analyzed by an unpaired t test. Careers. Draws on findings in a range of scientific disciplines to demonstrate how chronic fear in early childhood can be linked to common adult health issues, sharing illuminating case studies to reveal how compromises to an overworked fight-or ... And cancer is beaten at every stage. There are numerous success stories related to fenbendazole but not nearly as many associated with mebendazole, that I could find. designed research, analyzed data, supervised research, and wrote the manuscript; and all authors reviewed and edited the paper. Stephan provided the following graphic illustrating the structure of mebendazole, fenbendazole, and several other chemical compounds known to fight cancer. Comparative evaluation of acute toxicity of ivermectin by two methods after single subcutaneous administration in rats. This is only anecdotal and subjective evidence that fenbendazole is better but it is what I have to work with. Four (OCI-AML2), 5 (MDAY-D2), or 7 (K562) days after injection, once tumors were palpable, mice were treated with ivermectin (IVM; 3 mg/kg) by oral gavage in water or vehicle control (n = 10 per group) for 10 days (K562) or 8 doses over 10 days (OCI-AML2). (D) OCI-AML2 cells were treated simultaneously with ivermectin (3μM), the ROS scavenger, NAC (5μM), or both for 30 hours, and STAT1A and STAT1B expression was assessed as described for panel A. Ivermectin plays a role in several biological mechanisms, therefore it could serve as a potential candidate in the treatment of a wide range of viruses including COVID-19 as well as other types of positive-sense single-stranded RNA viruses. Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. For sure are sulfur molecules stronger in affecting microtubules. doi: 10.1016/j.mjafi.2021.06.002. The patient should be advised that treatment with ivermectin does not kill the adult Onchocerca parasites, and therefore repeated follow-up and retreatment is usually necessary. Data represent the mean ± SD fold change in ROS production compared with each of their buffer treated controls for experiments performed in triplicate. Dr. Ben Williams is another amazing cancer success story. A randomized, double-blind, placebo-controlled trial was conducted to determine the rapidity of viral clearance and saf … Having identified ivermectin in our chemical screens, we tested the effects of ivermectin on cell growth and viability in a panel of 5 leukemia cell lines. Prayers Answered!!! Moreover, the leukemia cells in the peripheral blood were controlled while receiving ivermectin. So at this point, we have two cases that partially attribute mebendazole to the defeat of two very different cancer types (I define defeat of cancer by long-term remission). Would you like email updates of new search results? From these screens, we identified ivermectin, a derivative of avermectin B1 that is licensed for the treatment of the parasitic infections, strongyloidiasis and onchocerciasis, but is also effective against other worm infestations. Within 24 hours of infection, there was a … Therefore, we initiated a phase 1 study to evaluate the dose-limiting toxicity, maximum tolerated dose, and recommended phase 2 dose of clioquinol in patients with relapsed or refractory hematologic malignancies (ClinicalTrials.gov ID NCT00963495). Accessibility However, at higher concentrations, ivermectin also activates mammalian chloride channels.7  Mammalian chloride channels broadly fall into 5 classes, based on their regulation: cystic fibrosis transmembrane conductance regulator, which is activated by cyclic AMP dependent phosphorylation; calcium-activated chloride channels; voltage-gated chloride channels (ClCs); ligand-gated chloride channels (γ-aminobutyric acid and glycine-activated); and volume-regulated chloride channels. Indo-1 AM, 6-methoxy-N-(3-sulfopropyl) quinolinium (SPQ), carboxydichlorofluorescein diacetate (Carboxy H2DCF-DA), 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl benzimidazlycarbocyanine iodide (JC1), and bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC4(3)) were all purchased from Invitrogen Canada. is a Canadian Institutes of Health Research postdoctoral fellow, and holds a postdoctoral research fellowship from the Multiple Myeloma Research Foundation and Millennium Pharmaceuticals. An Australian drug known as Ivermectin, which is already in use throughout the world to treat parasitic conditions, is showing great results in killing coronavirus in studies involving patients, according to Sky News. Mebendazole is traditionally used to kill parasitic worms and other parasites living inside the body. AAPS J. Cobalt chloride decreases EC-SOD expression through intracellular ROS generation and p38-MAPK pathways in COS7 cells. Phase II study of ketoconazole combined with weekly doxorubicin in patients with androgen-independent prostate cancer. One hundred fifty genes were deregulated > 4-fold at both time points (33 underexpressed; 117 overexpressed), compared with control. Data represent the mean ± SD fold change in ROS production compared with control. Biophys Chem. Once tumors were established, mice were treated with ivermectin (7 mg/kg) intraperitoneally or vehicle control for 5 days (n = 3 per group). 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